Biochemie Methodenkurs


Course Outline   Click on the title to link to the procedure.
  1.  Immunological detection methods
  2.  Enzyme kinetics
  3.  Isoelectric focusing
  4.  Electrophoresis
  5.  Ion-exchange chromatography
  6.  Protein fractionation
  7.  Radioactivity
  8.  Gel chromatography
  9.  Pigment analysis and UV spectroscopy

*  Lab occurs every Thursday in BioII/III, HS 2, EG.
*  All work will be done in pairs; 1 report to be handed in for each pair.
*  Keep an eye on anything hot or flammable! Know where the fire extinguisher and the first aid kit are!
*  Wear protective clothing!
*  Eating, drinking, smoking in the lab are forbidden!
*  Don't pipette with your mouth! Use the bulbs provided!
*  Wear goggles!
*  Wear gloves! Don't touch door handles, pipettes, ANYTHING with contaminated gloves!
*  Be careful with acids and bases!
*  With with organic solvents in the fumehood!
*  Clean everything after working with it!
*  If you have questions, ask the TA before continuing!
     Note from Kyra: This is a key difference between OGP and this course.

Notes in teal denote Kyra's English-to-English translation.
Parallel course - Bchm techniques at Michigan Tech U                                                                                                                                                                                          

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Copyright Misty Rious Productions, December 2002.